Determination Genotype
genotyping process of elucidating genotype of individual biological assay. known genotypic assay, techniques include pcr, dna fragment analysis, allele specific oligonucleotide (aso) probes, dna sequencing, , nucleic acid hybridization dna microarrays or beads. several common genotyping techniques include restriction fragment length polymorphism (rflp), terminal restriction fragment length polymorphism (t-rflp), amplified fragment length polymorphism (aflp), , multiplex ligation-dependent probe amplification (mlpa).
dna fragment analysis can used determine such disease causing genetics aberrations microsatellite instability (msi), trisomy or aneuploidy, , loss of heterozygosity (loh). msi , loh in particular have been associated cancer cell genotypes colon, breast , cervical cancer.
the common chromosomal aneuploidy trisomy of chromosome 21 manifests down syndrome. current technological limitations typically allow fraction of individual s genotype determined efficiently.
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